Uncharged Helical Modular Polypeptide Hydrogels for Cellular Scaffolds.

TitleUncharged Helical Modular Polypeptide Hydrogels for Cellular Scaffolds.
Publication TypeJournal Article
Year of Publication2015
AuthorsAhrens, CC, M Welch, E, Griffith, LG, Hammond, PT
Date Published2015 Dec 14

Grafted synthetic polypeptides hold appeal for extending the range of biophysical properties achievable in synthetic extracellular matrix (ECM) hydrogels. Here, N-carboxyanhydride polypeptide, poly(γ-propargyl-l-glutamate) (PPLG) macromers were generated by fully grafting the "clickable" side chains with mixtures of short polyethylene glycol (PEG) chains terminated with inert (-OH) or reactive (maleimide and/or norbornene) groups, then reacting a fraction of these groups with an RGD cell attachment motif. A panel of synthetic hydrogels was then created by cross-linking the PPLG macromers with a 4-arm PEG star molecule. Compared to well-established PEG-only hydrogels, gels containing PPLG exhibited dramatically less dependence on swelling as a function of cross-link density. Further, PPLG-containing gels, which retain an α-helical chain conformation, were more effective than standard PEG gels in fostering attachment of a human mesenchymal stem cell (hMSC) line for a given concentration of RGD in the gel. These favorable properties of PPLG-containing PEG hydrogels suggest they may find broad use in synthetic ECM.

Alternate JournalBiomacromolecules
PubMed ID26461932
Grant ListR01 EB010246-03 / EB / NIBIB NIH HHS / United States
U54-CA112967 / CA / NCI NIH HHS / United States