Title | Tailoring chimeric ligands for studying and biasing ErbB receptor family interactions. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Krueger, AT, Kroll, C, Sanchez, E, Griffith, LG, Imperiali, B |
Journal | Angew Chem Int Ed Engl |
Volume | 53 |
Issue | 10 |
Pagination | 2662-6 |
Date Published | 2014 Mar 3 |
ISSN | 1521-3773 |
Abstract | Described is the development and application of a versatile semisynthetic strategy, based on a combination of sortase-mediated coupling and tetrazine ligation chemistry, which can be exploited for the efficient incorporation of tunable functionality into chimeric recombinant proteins. To demonstrate the scope of the method, the assembly of a set of bivalent ligands, which integrate members of the epidermal growth factor (EGF) ligand family, is described. By using a series of bivalent EGFs with variable intraligand spacing, the differences in structure were correlated with the ability to bias signaling in the ErbB receptor family in a cell motility assay. Biasing away from EGFR-HER2 dimerization with a bivalent EGF was observed to reduce cell motility in an intraligand distance-dependent fashion, thus demonstrating the utility of the approach for acutely perturbing receptor-mediated cell signaling pathways. |
DOI | 10.1002/anie.201307869 |
Alternate Journal | Angew. Chem. Int. Ed. Engl. |
PubMed ID | 24481645 |
Grant List | 5-T32-ES007020 / ES / NIEHS NIH HHS / United States R01 DE019523 / DE / NIDCR NIH HHS / United States R01DE019523-13 / DE / NIDCR NIH HHS / United States T32 ES007020 / ES / NIEHS NIH HHS / United States U54 CA112967 / CA / NCI NIH HHS / United States U54-CA112967 / CA / NCI NIH HHS / United States |