| Title | Protein Engineering and Selection Using Yeast Surface Display. |
| Publication Type | Journal Article |
| Year of Publication | 2015 |
| Authors | Angelini, A, Chen, TF, de Picciotto, S, Yang, NJ, Tzeng, A, Santos, MS, Van Deventer, JA, Traxlmayr, MW, K Wittrup, D |
| Journal | Methods Mol Biol |
| Volume | 1319 |
| Pagination | 3-36 |
| Date Published | 2015 |
| ISSN | 1940-6029 |
| Keywords | Cell Surface Display Techniques, Combinatorial Chemistry Techniques, Flow Cytometry, Magnets, Mutagenesis, Peptide Library, Protein Binding, Proteins, Saccharomyces cerevisiae |
| Abstract | Yeast surface display is a powerful technology for engineering a broad range of protein scaffolds. This protocol describes the process for de novo isolation of protein binders from large combinatorial libraries displayed on yeast by using magnetic bead separation followed by flow cytometry-based selection. The biophysical properties of isolated single clones are subsequently characterized, and desired properties are further enhanced through successive rounds of mutagenesis and flow cytometry selections, resulting in protein binders with increased stability, affinity, and specificity for target proteins of interest. |
| DOI | 10.1007/978-1-4939-2748-7_1 |
| Alternate Journal | Methods Mol. Biol. |
| PubMed ID | 26060067 |
| Grant List | 1 U54 CA112967 / CA / NCI NIH HHS / United States |
