Title | Characterization of the expression of the pro-metastatic Mena(INV) isoform during breast tumor progression. |
Publication Type | Journal Article |
Year of Publication | 2016 |
Authors | Oudin, MJ, Hughes, SK, Rohani, N, Moufarrej, MN, Jones, JG, Condeelis, JS, Lauffenburger, DA, Gertler, FB |
Journal | Clin Exp Metastasis |
Volume | 33 |
Issue | 3 |
Pagination | 249-61 |
Date Published | 2016 Mar |
ISSN | 1573-7276 |
Abstract | Several functionally distinct isoforms of the actin regulatory Mena are produced by alternative splicing during tumor progression. Forced expression of the Mena(INV) isoform drives invasion, intravasation and metastasis. However, the abundance and distribution of endogenously expressed Mena(INV) within primary tumors during progression remain unknown, as most studies to date have only assessed relative mRNA levels from dissociated tumor samples. We have developed a Mena(INV) isoform-specific monoclonal antibody and used it to examine Mena(INV) expression patterns in mouse mammary and human breast tumors. Mena(INV) expression increases during tumor progression and to examine the relationship between Mena(INV) expression and markers for epithelial or mesenchymal status, stemness, stromal cell types and hypoxic regions. Further, while Mena(INV) robustly expressed in vascularized areas of the tumor, it is not confined to cells adjacent to blood vessels. Altogether, these data demonstrate the specificity and utility of the anti-Mena(INV)-isoform specific antibody, and provide the first description of endogenous Mena(INV) protein expression in mouse and human tumors. |
DOI | 10.1007/s10585-015-9775-5 |
Alternate Journal | Clin. Exp. Metastasis |
PubMed ID | 26680363 |
PubMed Central ID | PMC4777680 |
Grant List | P01 CA100324 / CA / NCI NIH HHS / United States P30 CA013330 / CA / NCI NIH HHS / United States R01 CA150344 / CA / NCI NIH HHS / United States R01 GM058801 / GM / NIGMS NIH HHS / United States U54 CA112967 / CA / NCI NIH HHS / United States |