NetworKIN: a resource for exploring cellular phosphorylation networks.

TitleNetworKIN: a resource for exploring cellular phosphorylation networks.
Publication TypeJournal Article
Year of Publication2008
AuthorsLinding, R, Jensen, LJ, Pasculescu, A, Olhovsky, M, Colwill, K, Bork, P, Yaffe, MB, Pawson, T
JournalNucleic Acids Res
IssueDatabase issue
Date Published2008 Jan
KeywordsAmino Acid Sequence, Consensus Sequence, Databases, Protein, Humans, Internet, Phosphoproteins, Phosphorylation, Protein Kinases, Proteomics, User-Computer Interface

Protein kinases control cellular responses by phosphorylating specific substrates. Recent proteome-wide mapping of protein phosphorylation sites by mass spectrometry has discovered thousands of in vivo sites. Systematically assigning all 518 human kinases to all these sites is a challenging problem. The NetworKIN database ( integrates consensus substrate motifs with context modelling for improved prediction of cellular kinase-substrate relations. Based on the latest human phosphoproteome from the Phospho.ELM and PhosphoSite databases, the resource offers insight into phosphorylation-modulated interaction networks. Here, we describe how NetworKIN can be used for both global and targeted molecular studies. Via the web interface users can query the database of precomputed kinase-substrate relations or obtain predictions on novel phosphoproteins. The database currently contains a predicted phosphorylation network with 20,224 site-specific interactions involving 3978 phosphoproteins and 73 human kinases from 20 families.

Alternate JournalNucleic Acids Res.
PubMed ID17981841
PubMed Central IDPMC2238868
Grant ListGM60594 / GM / NIGMS NIH HHS / United States
U54-CA112967 / CA / NCI NIH HHS / United States