| Title | Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator. |
| Publication Type | Journal Article |
| Year of Publication | 2011 |
| Authors | Chen, C-H, Sarkar, A, Song, Y-A, Miller, MA, Kim, SJ, Griffith, LG, Lauffenburger, DA, Han, J |
| Journal | J Am Chem Soc |
| Volume | 133 |
| Issue | 27 |
| Pagination | 10368-71 |
| Date Published | 2011 Jul 13 |
| ISSN | 1520-5126 |
| Keywords | Animals, Cells, Cultured, Fibroblasts, Matrix Metalloproteinases, Mice, Microfluidic Analytical Techniques, Microfluidics, Sensitivity and Specificity |
| Abstract | We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample volume used. |
| DOI | 10.1021/ja2036628 |
| Alternate Journal | J. Am. Chem. Soc. |
| PubMed ID | 21671557 |
| PubMed Central ID | PMC3165005 |
| Grant List | GM68762 / GM / NIGMS NIH HHS / United States P50 GM068762-08 / GM / NIGMS NIH HHS / United States R01 EB010246 / EB / NIBIB NIH HHS / United States R01 GM081336-04 / GM / NIGMS NIH HHS / United States R01-EB010246 / EB / NIBIB NIH HHS / United States R01-GM081336 / GM / NIGMS NIH HHS / United States U54-CA112967 / CA / NCI NIH HHS / United States |
